1061 / 2024-09-20 10:12:42

Deoxygenation enhances photosynthetic performance and increases N2 fixation in the marine cyanobacterium Trichodesmium under elevated pCO2

growth rate,nitrogen fixation,photosynthesis,respiration,Trichodesmium erythraeum

Session 9 - Global Ocean Changes: Regional Processes and Ecological Impacts

Effects of changed levels of dissolved O₂ and CO₂ on marine primary producers are of general concern with respect to ecological effects of ongoing ocean deoxygenation and acidification as well as upwelled seawaters. We investigated the response of the diazotroph Trichodesmium erythraeum IMS 101 after it had acclimated to lowered pO₂ (~60 μM O₂) and/or elevated pCO₂ levels (HC, ~32 μM CO₂) for about 20 generations. Our results showed that reduced O₂ levels decreased dark respiration significantly, and increased the net photosynthetic rate by 66% and 89% under the ambient (AC, ~13 μM CO₂) and the HC, respectively. The reduced pO₂ enhanced the N₂ fixation rate by ~139% under AC and only by 44% under HC, respectively. The N₂ fixation quotient, the ratio of N₂ fixed per O₂ evolved, increased by 143% when pO₂ decreased by 75% under the elevated pCO₂. Meanwhile, particulate organic carbon and nitrogen quota increased simultaneously under reduced O₂ levels, regardless of the pCO₂ treatments. Nevertheless, changed levels of O₂ and CO₂ did not bring about significant changes in the specific growth rate of the diazotroph. Such inconsistency was attributed to the daytime positive and nighttime negative effects of both lowered pO₂ and elevated pCO₂ on the energy supply for growth. Our results suggest that Trichodesmium decrease its dark respiration by 5% and increase its N₂-fixation by 49% and N₂-fixation quotient by 30% under future ocean deoxygenation and acidification with 16% decline of pO₂ and 138% rise of pCO₂ by the end of this century.